HNMR was obtained of the product after it was re-dissolved with ether, dried with MgSO4, and vacuumed again to have a net weight of 15mg. There is no carbonyl peak that I can see and the NMR seems skewed. There is an expanded version as well.
Discussion
Based on the lack of an aldehyde proton in the 9-10 ppm range, it does not look like any aldehyde was isolated.
Conclusion
Based on the NMR, the aldehyde was not produced however the reaction will be attempted once more.
Log
2006-06-22
16:55] turn on hot plate to heat glycerol
17:10] put phosphoric solution in 10mL vial and then in heated glycerol bath
17:23] solution heated to 120C, taken off of heat and cooled for 30seconds. added to 36mL distilled water.
19:00] Saturated with NaCl(didn't filter)
19:13] Poured solution without excess NaCl into a separatory funnel. Extracted with 17mL anhydrous ether
19:20] Extracted with 10mL anhydrous ether
19:24] Extracted with 10mL ether
19:30] Performed TLC 1:1 methylene chloride hexanes (no movement), pure methylene chloride (no movement), pure methanol (some movement)
Ninhydrin and Ansaldehyde stains give little information.
2006-06-23
rotovaped ether extracts
2006-06-26
vacuumed off remains from rotovap. wt=15mg
dry product would not dissolve in methylene chloride but did disolve in methanol
TLC of 1:1 methylene chloride in hexanes showed nothing. Methanol had same movement
Objective
To convert adrenaline to a catechol aldehyde using acid catalysis. How this fits into the synthesis of anti-malarials is described here.Procedure
Taken from Preparation and Properties of p-Hydroxyphenylacetaldehyde and 3-Methoxy-4-Hydroxyphenylacetaldehyde by Jay H. Robbins Archives of Biochemistry and Biophysics.A solution of adrenaline (217 mg, 1.18 mmol) in 85% phosphoric acid (6 mL) was heated in a 12mL Was it 10mL or 12mL? Compare to log. vial to 120C in a glycerol bath then removed from heat and allowed to cool for 30 seconds then added to water (36 mL) and then saturated with NaCl, followed by extraction with ether (17mL, 10mL, 10mL). Add in the TLC preformed at 19:30 on 06/22, as well as the NMR that was taken.
Results
Video of set upCharacterization
Two stains were done without a TLC run Ninhydrin stain Anisaldehyde stainRun in pure methanol visible with iodine.
HNMR was obtained of the product after it was re-dissolved with ether, dried with MgSO4, and vacuumed again to have a net weight of 15mg. There is no carbonyl peak that I can see and the NMR seems skewed. There is an expanded version as well.
Discussion
Based on the lack of an aldehyde proton in the 9-10 ppm range, it does not look like any aldehyde was isolated.Conclusion
Based on the NMR, the aldehyde was not produced however the reaction will be attempted once more.Log
2006-06-22
16:55] turn on hot plate to heat glycerol17:10] put phosphoric solution in 10mL vial and then in heated glycerol bath
17:23] solution heated to 120C, taken off of heat and cooled for 30seconds. added to 36mL distilled water.
19:00] Saturated with NaCl(didn't filter)
19:13] Poured solution without excess NaCl into a separatory funnel. Extracted with 17mL anhydrous ether
19:20] Extracted with 10mL anhydrous ether
19:24] Extracted with 10mL ether
19:30] Performed TLC 1:1 methylene chloride hexanes (no movement), pure methylene chloride (no movement), pure methanol (some movement)
Ninhydrin and Ansaldehyde stains give little information.
2006-06-23
rotovaped ether extracts2006-06-26
vacuumed off remains from rotovap. wt=15mgdry product would not dissolve in methylene chloride but did disolve in methanol
TLC of 1:1 methylene chloride in hexanes showed nothing. Methanol had same movement
Tags
AdrenalineInChI=1/C9H13NO3/c1-10-5-9(13)6-2-3-7(11)8(12)4-6/h2-4,9-13H,5H2,1H3DOPAL InChI=1/C8H8O3/c9-4-3-6-1-2-7(10)8(11)5-6/h1-2,4-5,10-11H,3H2